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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... graded transactivation potential Nucleic Acids Res 25, 2723–2729 13 Akagi K, Kanai M, Saya H, Kozu T & Berns A (2001) A novel tetracycline-dependent transactivator with E2F4 transcriptional activation ... CGGGCCCGGGATCCATC gccacc ATGG TGA; the 3¢ interface is CAAGTAAA GCGGCCGC For pEBTetD ⁄ eGFP, the 5¢ interface is identical; the 3¢ interface is CAAGTAAA GCGGCCGCGG Cell culture, transfection, and flow ... and with a GAPDH probe (cf Fig 2) Relative background transcription was calculated from the ratios of signals for transporter mRNA and GAPDH mRNA With OCT1, OCT2, and CAT4 both vectors were analyzed...
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Báo cáo Y học: LRP130, a protein containing nine pentatricopeptide repeat motifs, interacts with a single-stranded cytosine-rich sequence of mouse hypervariable minisatellite Pc-1 docx
... respectively a- tubulin was used as a marker for cytoplasmic extraction The same membrane was used for immunoblot analysis with aF-N, aF-C and anti- (a- tubulin) Ig Lanes and 4, whole cell lysate; lanes and ... accordance with the embryonal develop- Nuclear and cytoplasmic fractions were prepared from NIH3T3 cells and analyzed by immunoblot analysis As shown in Fig 3A, a major band with a molecular mass ... and the first strand cDNAs were synthesized with avian myeblastoma virus (AMV) reverse transcriptase (Takara Biomedicals) at 45 °C for 60 Partial cDNA fragments for the mLRP130 were amplified with...
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Báo cáo y học: "Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodies" ppsx
... with an offset of two amino acids (13 amino acid overlap) Each arginine-containing peptide was synthesized as three variants, one with natural arginine, one with sDMA and one with asymmetrical ... dimethylated arginine residues for epitopes assay Assay performance characteristics of the anti-SmD3 peptide (SMP) assay (a) Intra-assay and interassay variability, (b) linearity, and (c) receiver operating ... 41.3 and 13.9 units, respectively R24 Correlation with racial and clinical parameters When correlating autoantibody specificities with race, there was a statistically significant association of autoantibodies...
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Báo cáo y học: "Phase delaying the human circadian clock with a single light pulse and moderate delay of the sleep/dark episode: no influence of iris color" potx
... African Americans had larger phase advances than Caucasians Because we found that African Americans had a shorter tau than Caucasians, which would augment phase advances relative to the Caucasians ... manuscript we also re-analyzed data from our previous phase-advancing study with daily light pulses [42] that included both light and dark-eyed Caucasians as well as dark-eyed African Americans, and thus ... circadian adaptation J Biol Rhythms 1997, 12:5-15 Yang CM, Spielman AJ, D'Ambrosio P, Serizawa S, Nunes J, Birnbaum J: A single dose of melatonin prevents the phase delay associated with a delayed...
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application of real coded genetic algorithm for ship hull surface fitting with a single non-uniform b-spline surface
... Chiyokura and Kimura (1984) As a result, boundary interpolating patch has several drawbacks for modification a large number of small surfaces that are bonded together to create a larger surface 2.2 ... rectangular patches and triangular patches A flexible CAD system should perform a good quality of shape and an interactive manipulation The B-spline vertex point modification actually works with ... tolerance Fitting process is also expected that the surface is obtained in a reasonable way with high quality of shape Many previous researches approached to automated B-spline surface modeling mathematically...
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Narrowband photon pairs from a cold atomic vapour for interfacing with a single atom
... work with him and Sandako while doing HOM measurements Gleb, for always teasing me I still miss that Dzmitry, for his great ideas One can approach him anytime and any day and he is always ready ... available in the market 87 Rb is a naturally occurring isotope of Rubidium with atomic number 37 It has a natural abundance of 28%, mass of 86.9 amu with a nuclear spin I of 3/2 Rubidium has another ... with relevant transitions used for cooling the atoms is indicated (Right) Magneto-Optical Trap set up: a glass cuvette attached to a vacuum chamber, quadruple coils and circular polarized beams...
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Comparison of the obstetric anesthesia activity index with total delivery numbers as a single denominator of workload demand in Israeli maternity units doc
... anesthesia activity index (OAAI) The majority of anesthesia workload in the labor ward comprises epidural labor analgesia and cesarean delivery The OAAI is a formula composite comprising data ... cesarean anesthesia to the total OAAI in individual hospitals Data for 2005 (left) and 2007 (right) The OAAI correlated with epidural rates and cesarean rates more closely than did the annual ... the OAAI (OAAI EPI and OAAI CD) was also calculated as follows: OAAICD/EPI = ( no of cesareans per yr *1.5 ) / ( no of epidurals per yr *0.75) (2) Statistical methods Quantitative variables are...
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Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf
... greater catalytic efficiency as a reductase than as a dehydrogenase The catalytic efficiency of RDH13 as a retinaldehyde reductase is significantly lower than that of a related protein RDH11, primarily ... incubated at 28 °C for 3–4 days The mitochondrial fraction was isolated as described above, and then solubilized with 15 mm DHPC (Avanti Polar Lipids, Alabaster, AL, USA) in a buffer containing ... comparing their peak areas with a calibration curve constructed from the peak areas of a series of standards Determination of kinetic constants The apparent Km values for the reduction of retinaldehyde...
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Báo cáo khoa học: Biosynthesis of platelet glycoprotein V expressed as a single subunit or in association with GPIb-IX doc
... Immunoprecipitation and SDS/PAGE Materials and cell lines The mAbs V.1 and V.5 against GPV, ALMA.12 against GPIba, ALMA.16 against GPIX and RAM.1 against GPIbb, were developed in our laboratory [19] ... mass forms Parallel analysis of the supernatants revealed a positive signal starting at 60 of chase and having a molecular mass (82 kDa) consistent with a fully mature form This band was not observed ... Strassel, C., Baas, M.J., Salamero, J., ChasserotGolaz, S., Cazenave, J.P., De La Salle, C & Lanza, F (2001) Biosynthesis and intracellular post-translational processing of normal and mutant platelet...
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Báo cáo hóa học: "Dynamic changes in cellular infiltrates with repeated cutaneous vaccination: a histologic and immunophenotypic analysis" pot
... the principal investigator and participated in the data collection and analysis and preparation of the manuscript All authors have read and approved the final manuscript of this paper Competing ... that adjuvants that could increase Th1 cytokines may be valuable In particular, IL-12 and adjuvants that induce IL-12 production may be advantageous immune modulators by enhancing Th1 polarization ... Bland KI, Murray D, Robinson WA, Flaherty L, Richards JM, Bartolucci AA, Rosen L: Surgical adjuvant active specific immunotherapy for patients with stage III melanoma: the final analysis of data...
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báo cáo hóa học: " Psychometric properties of a single-item scale to assess sleep quality among individuals with fibromyalgia" potx
... clinical trials During the baseline phase, study patients had to have an average daily diary pain score of at least (within the last days) on a numeric rating scale ranging from ("no pain") to ... medication up to and including Day1 If fewer than scores are available then baseline consists of all scores that are available Page of (page number not for citation purposes) Health and Quality ... Quality Scale were obtained from all available study patients across all treatment groups Test re-test reliability of the Sleep Quality scores were evaluated using pre-treatment data Intraclass...
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báo cáo hóa học: " Boosting with intranasal dendrimeric Aβ1–15 but not Aβ1–15 peptide leads to an effective immune response following a single injection of Aβ1–40/42 in APP-tg mice" ppt
... has been demonstrated that non-B cell mechanisms may also have a role in clearing cerebral A [8] A multi-center A vaccine human clinical trial (AN1792) was initiated but was suspended when approximately ... each treatment group Statistical significance was determined using Kruskal-Wallis nonparametric one-way ANOVA (B) Immunostaining with the anti -A rabbit polyclonal antibody, R1282, demonstrates ... brain These data provide further data towards the safety and efficacy of dAβ1–15 immunization as a potential A vaccine, although it is acknowledged that the adverse events observed in the AN1792...
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Báo cáo toán học: " Shrinking projection algorithms for equilibrium problems with a bifunction defined on the dual space of a Banach space" doc
... 17 Takahashi, W: Nonlinear Functional Analysis-Fixed Point Theory and Its Applications Yokohama Publishers (2000) 18 Xu, HK: Inequalities in Banach spaces with applications Nonlinear Anal (TMA) ... and motivated by Ceng et al [2], Takahashi and Zembayashi [14], Takahashi and Zembayashi [9], the main aim of this paper is to introduce and investigate a new iterative method for finding a solution ... 11 Honda, T, Takahashi, W, Yao, JC: Nonexpansive retractions onto closed convex cones in Banach spaces Taiwanese J Math 14, 1023–1046 (2010) 12 Kohsaka, F, Takahashi, W: Generalized nonexpansive...
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báo cáo hóa học:" Research Article ´ ¨ Sharp Constants of Brezis-Gallouet-Wainger Type Inequalities with a Double Logarithmic Term on Bounded Domains in Besov and Triebel-Lizorkin Spaces" pdf
... homogeneous and logarithmic functions,” Communications in Mathematical Analysis, vol 9, no 2, pp 51–66, 2010 K Morii, T Sato, and H Wadade, “Br´ zis-Gallou¨ t-Wainger type inequality with a double logarithmic ... Semigroups and Optimal Regularity in Parabolic Problems, vol 16 of Progress in Nonlinear Differential Equations and their Applications, Birkh¨ user, Basel, Switzerland, 1995 a 16 S Ibrahim, M Majdoub, and ... zis-Gallou¨ t-Wainger type inequality for Besov-Morrey spaces,” Studia Mathematica, e e vol 196, no 1, pp 91–101, 2010 K Morii, T Sato, and Y Sawano, “Certain identities on derivatives of radial homogeneous...
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Báo cáo hóa học: " Research Article Orlicz Sequence Spaces with a Unique Spreading Model" pdf
... University, Tianjin, China, 2007 G Androulakis, E Odell, Th Schlumprecht, and N Tomczak-Jaegermann, “On the structure of the spreading models of a Banach space, ” Canadian Journal of Mathematics, vol 57, ... Odell, and B Sari, “Lattice structures and spreading models,” Israel Journal of Mathematics, vol 161, pp 387–411, 2007 A Brunel and L Sucheston, “On B-convex Banach spaces,” Mathematical Systems ... SPw hM and CM,1 coincide That is, SPw hM Journal of Inequalities and Applications Orlicz Sequence Spaces with Equivalent Spreading Models Definition 3.1 see Let xn be a normalized Schauder basis...
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Báo cáo hóa học: " Research Article Equivalent Solutions of Nonlinear Equations in a Topological Vector Space with a Wedge" doc
... in Applied Mathematics, Heldermann, Berlin, Germany, 1989 [8] M G Kre˘n and M A Rutman, “Linear operators leaving invariant a cone in a Banach space, ” ı American Mathematical Society Translations, ... Journal of Inequalities and Applications the solvability of abstract second-kind equations of type (1.1) in a Banach space with a normal cone, we refer, for example, to [2] Definitions, notation, and ... Corollary 4.5(b) can be applied As it was said above (see Remark 4.3), in the case where K is a cone, Theorems 4.1, 4.2, and their corollaries guarantee that (1.1) has at most one solution Note again...
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Báo cáo hóa học: "ON MULTIVALUED NONLINEAR VARIATIONAL INCLUSION PROBLEMS WITH (A,η)-ACCRETIVE MAPPINGS IN BANACH SPACES" docx
... (H,η)-accretive operators and approximating solutions for systems of variational inclusions in Banach spaces, to appear in Nonlinear Analysis [3] Y.-P Fang and N.-J Huang, H-monotone operator and ... Iterative algorithm for a system of variational inclusions involving H-accretive operators [6] in Banach spaces, Acta Mathematica Hungarica 108 (2005), no 3, 183–195 [7] Y.-P Fang, N.-J Huang, and ... H.-Y Lan, Y J Cho, and R U Verma, On nonlinear relaxed cocoercive variational inclusions involving (A, η)-accretive mappings in Banach spaces, to appear in Computers & Mathematics with Applications...
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Báo cáo lâm nghiệp: "Do trees use reserve or newly assimilated carbon for their defense reactions? A 13 C labeling approach with young Scots pines inoculated with a bark-beetle-associated fungus (Ophiostoma brunneo ciliatum" pptx
... needle area was measured with a leaf area meter (Delta-T Devices, Cambridge, UK) Once Ψwp had reached a threshold of around –2 MPa (after approx 10 days), saplings were watered to field capacity and ... withholding irrigation (Fig 1) Every second day, predawn needle water potential (Ψwp ) was measured with a Scholander pressure chamber, and gas exchange of a current year twig with a 4L portable ... well-watered controls (Figs 2a and 2b) Daily water use was about 0.45 L day−1 from an available soil water reserve of about L Predawn needle water potential (Ψwp ) fluctuated around −0.34 MPa throughout...
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